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Supplementary Information for:
P21 Cip1 is a critical mediator of the
cytotoxic action of thymidylate synthase (TS) inhibitors in colorectal carcinoma cells
James I. Geller 1,4, Kinga Szekely-Szucs 1, Istvan Petak 2, Belinda Doyle 1, and Janet A. Houghton 1,3
1 Division of Molecular Therapeutics, Department of Hematology-Oncology, St Jude Children’s Research Hospital, 332 North Lauderdale, Memphis, TN 38105;
2Semmelweis University and 1 st Institute of Pathology and Experimental Cancer Research, Budapest, Hungary.
Support: Supported by NIH Awards RO1 CA 32613, 4T32-CA70089, Cancer Center Support (CORE) Grant CA 21765, and by the American Lebanese Syrian Associated Charities.
3Corresponding author:
Janet A. Houghton Ph.D.
Division of Molecular Therapeutics
Department of Hematology-Oncology
St. Jude Children's Research Hospital
332 North Lauderdale
Memphis , TN 38105.
Tel: (901)495-3456
Fax: (901)495-3966
E-mail: janet.houghton@stjude.org
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Gene expression profiles in HT29 cells following thymineless stress
A - 5Fluorouracil/Leucovorin treatment
H1324P and H1326P: Control labeled with Cy3 (F532) and 5Fluorouracil treated sample labeled with Cy5 (F635)
H1323P and H1328P: Dye flips i.e. Control labeled with Cy5 (F635) and 5Fluorouracil treated sample labeled with Cy3 (F532)
- Upregulated Genes
- Downregulated Genes
B - ZD9331 treatment
H1322P and H1329P: Control labeled with Cy3 (F532) and ZD9331 treated sample labeled with Cy5 (F635)
H1321P and H1330P: Dye flips i.e. Control labeled with Cy5 (F635) and 5Fluorouracil treated sample labeled with Cy3 (F532)
Up and downregulated genes identified by filtering out unreliable data and then performing global normalization, log transformation and t-test based significance analysis. Significantly differential genes were then separated on the basis of log(fold change). Genes with a p-value < 0.05 and an average fold change of 50% were selected.
- Upregulated Genes
- Downregulated Genes
Data analyzed by Deepak Kaushal, PhD, Scientist I, Functional Genomics Laboratory, Hartwell Center for Bioinformatics and Biotechnology, St Jude Children’s Research Hospital , Memphis TN.
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