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Supplementary Information for:

Gene-expression patterns in drug resistant acute lymphoblastic leukemia cells and response to treatment.
Amy Holleman, Meyling H. Cheok, Monique L. den Boer, Wenjian Yang, Anjo J.P. Veerman, Karin M. Kazemier, Deqing Pei, Cheng Cheng, Ching-Hon Pui, Mary V. Relling, Gritta E. Janka-Schaub, Rob Pieters, and William E. Evans

Supplemental Table 5 : Prediction accuracy using gene expression profiles for classification of drug resistant and sensitive acute lymphoblastic leukemia.

Prediction accuracy in randomly selected cohorts comprising 1/3 of the training set for each antileukemic agent using 30, 50 or 100 reselected top probe sets. The median prediction accuracy is shown with corresponding P-values and the 95 percent confidence interval (C.I.) for prednisolone (PRED), vincristine (VCR), asparaginase (ASP) and daunorubicin (DNR) (a) based on all patients and (b) for only patients with B-lineage acute lymphoblastic leukemia. The prediction accuracies were even higher (c, d), when genes were selected only once from the entire training set, and then used to predict sensitive or resistant phenotypes in randomly selected cohorts comprising 1/3 of the population.

a. All patients

 

N

accuracy
[%]

95%
C.I.

P-value

PRED

30

71

58-81

0.031

 

50

71

58-81

0.031

 

100

71

58-81

0.031

VCR

30

68

59-78

0.029

 

50

71

59-79

0.012

 

100

71

59-79

0.012

ASP

30

67

52-76

0.045

 

50

67

52-79

0.045

 

100

67

55-78

0.045

DNR

30

73

60-80

0.007

 

50

73

60-80

0.007

 

100

73

58-76

0.007

b. B-lineage

accuracy
[%]

95%
C.I.

P-value

75

63-88

0.025

75

63-88

0.025

75

67-87

0.025

74

62-85

0.010

76

65-85

0.004

76

65-85

0.004

71

60-83

0.018

71

57-83

0.018

71

60-80

0.018

76

65-85

0.004

76

65-85

0.004

76

67-84

0.004

 

c. All patients

 

N

accuracy
[%]

95%
C.I.

PRED

30

95

88-100

 

50

93

86-100

VCR

30

81

71-88

 

50

81

74-90

ASP

30

81

71-90

 

50

83

71-91

DNR

30

90

83-95

 

50

90

86-98

d. B-lineage

accuracy
[%]

95%
C.I.

98

90-100

98

90-100

81

71-88

81

71-91

83

71-90

86

74-93

95

90-100

95

90-100